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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Buletin Kimia VALENSI Jurnal Veteriner Jurnal Natur Indonesia JURNAL SAINS DAN TEKNOLOGI INDONESIA JURNAL TEKNOLOGI LINGKUNGAN Jurnal Penelitian Hasil Hutan The Journal of Pure and Applied Chemistry Research EduChemia (Jurnal Kimia dan Pendidikan) Indonesian Journal of Chemistry Marinade EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Molekul: Jurnal Ilmiah Kimia Jurnal Jamu Indonesia Asian Journal of Aquatic Sciences Jurnal Penelitian Hasil Hutan Makara Journal of Science Jurnal Kedokteran Hewan
Purwantiningsih Sugita
Departemen Kimia, Fakultas MIPA, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Veterinary Other

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Journal : Jurnal Jamu Indonesia

 1 
Aktivitas Antioksidan, Antibakteri, dan Toksisitas Ekstrak Kulit Batang Pohon Kesambi (Schleichera oleosa (Lour) Oken) Puspita Sari; Purwantiningsih Sugita; Adi Santoso
Jurnal Jamu Indonesia Vol. 4 No. 3 (2019): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1021.706 KB) | DOI: 10.29244/jji.v4i3.163

Abstract

Penelitian ini bertujuan untuk membandingkan aktivitas antioksidan, antibakteri, dan uji toksisitas dari berbagai jenis ekstrak kulit batang pohon kesambi (Schleichera oleosa (Lour)Oken). Tahapan penelitian meliputi proses esktraksi kulit batang kesambi dari kecamatan Mancak Serang dengan teknik maserasi bertingkat menggunakan 3 pelarut : Metanol, n-heksan, dan etil asetat. Ekstrak tersebut kemudian diuji aktivitas antioksidan, antibakteri, dan toksisitasnya. Hasil pengujian antioksidan dengan metode DPPH, antibakteri dengan metode difusi agar, dan uji toksisitas dengan metode BSLT didapat hasil penelitian menunjukkan bahwa ekstrak etil asetat memiliki aktivitas antioksidan (IC50 7,723 ppm) yang lebih baik dibandingkan dengan ekstrak metanol (IC50 7,801 ppm), dan ekstrak n-heksan (IC50 8,568 ppm). Aktivitas antibakteri menunjukkan adanya kemampuan untuk menghambat pertumbuhan bakteri Streptococcus aureus dibandingkan bakteri Escherichia coli pada konsentrasi 10000 ppm, dan hasil uji toksisitas menunjukkan bahwa ekstrak etil asetat memiliki aktivitas yang lebih baik (LC50 305,17 ppm) dibandingkan ekstrak n-heksan (LC50 374,96 ppm) dan ekstrak metanol (LC50 431,26 ppm).
Gelatin Extraction and Characterization from Femur Bones of Bovine and Porcine with Acid Process Purwantiningsih Sugita; Muhamad Rifai; Laksmi Ambarsari; Dyah Utami Cahyaning Rahayu; Hanhan Dianhar
Jurnal Jamu Indonesia Vol. 6 No. 1 (2021): Jurnal Jamu Indonesia
Publisher : Pusat Studi Biofarmaka Tropika LPPM IPB; Tropical Biopharmaca Research Center - Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v6i1.188

Abstract

Gelatin has been widely used as an additive in food industry pharmaceutical, and cosmetic. The similar physical appearance between bovine and porcine gelatin causes an issue for some communities like a Muslim due to awareness of halal food. This study aims to produce gelatin from femur bones of bovines with acid hydrolysis and their characteristics compared to standard gelatin of bovine and porcine. Bovine and porcine bones were soaked in 5% HCl for 10 days and every 2 days a HCl solution was replaced to get ossein. Ossein is hydrolyzed by gradual heating at 65, 75, and 85oC. Gelatin confirmed by the physico-chemical characters, FT-IR and analysis amino acid with HPLC.The results showed that the yield of bovine gelatin was 4.33%. The physico-chemical characters of bovine gelatin resulting from isolation and bovine gelatin standards are in conformity with porcine gelatin standards and meet the requirements of SNI 06-3735-1995 and GMIA. Therefore, bovine gelatin is specifically capable of substituting porcine gelatin for application in the pharmaceutical field. The FTIR spectrum of bovine gelatin shows the presence of amide A, amide I, amide II and amide III groups. The amino acid characters of gelatin were identified as glycine (13.57%) and proline (1.62%) for bovine gelatin and glycine (0.51%) and proline (0.09%) for porcine gelatin.
Co-Authors Achmad Sjachriza Achmad Sjahriza Ade Heri Mulyati Adi Santoso ADI SANTOSO Asep Hidayat Asep Hidayat Asep Saefurohman Auliya Ilmiawati Azwin Apriandi Azwin Apriandi Azwin Apriandi Bambang Srijanto Bambang Srijanto Bambang Srijanto Budi Arifin Cahyaningsih, Umi Cece Sumantri Dedi Duryadi Solihin Dian Susanthy Diana Widiastuti Dita Ariyanti dwi wahyono Dyah Iswantini Dyah Utami Cahyaning Rahayu Ellin Vina Setyowati Epi Taufik Ermin Katrin Winarno Ermin Katrin Winarno Fifia Zulti fithri amelia Ghozali, Ali Aulia Gustini Syahbirin Hanhan Dianhar Harjono - Hefni Effendi HENNY PURWANINGSIH Herdini . Irmanida Batubara Jaya Hardi Kiagus Dahlan Kurniawan, Yehezkiel Steven Kustiariyah Tarman LAKSMI AMBARSARI Latifah K. Darusman Lia Anriani Luthfan Irfana mahdi mubarok Mela Faradika Mersi Kurniati Muhamad Rifai Muhammad Fathurrahman Napthaleni , Novik Nurhidayat Nur Qadri Rasyid Okti Rachmawati Okti Rachmawati Pujiyati Pujiyati Pujiyati, Puspita Sari Rarah Ratih Adjie Maheswari Rini Siswati Asnel Siti Warnasih Sri Sugiarti Suminar S Achmadi Suminar Setiati Achmadi SUMINAR SETIATI ACHMADI Taher, Dharmawaty M. Tiltje Andretha Ransaleleh Tuti Wukirsari Tuti Wukirsari Uswatun Hasanah Wasmen Manalu Yuyu Yundhana Zainal Alim Mas’ud Zuniar Subastian Zuraida Hanum Zuraida Hanum